Fourth Entry e
I. What have you learned?
I have learned new techniques in the laboratory. I learned about how to improve some techniques that I already knew. I have learned that the research requires a lot of dedication.
II. What barriers and challenges you have to confront for began to realize you research?
The economy was a challenge because we have to work and deal with the materials that we have. For example is will be greater a gel of acrylamide than an agarose gel but the budget and the equipment don’t allow us that. Also a barrier has been the place where we sent our DNA for receive the sequences. Because we still waiting, and probably we have to make again mini preps because of the University that don’t sent us all the sequences of the DNA that we sent them.
III. How have you overcame the barriers and challenges?
With patience and acceptation of the reality of the research.
IV. What are do you expect for next semester?
I expect to have the sequence of the mutagenic DNA of GFP for made a comparison between the original and the mutagenic that we created. Also I expect to begin the characterization of the protein GFP.
April 26, 2008
Excelente. Que tengas un verano espectacular y recuerda someter lel informe, evaluaciones y certificacion de asistencia. exito!
Saludos, Militza! Soy el profesor Lorenzo Saliceti, del Recinto de Mayagüez.
En mi laboratorio utilizo GFP para el monitoreo de biorreactores. Es una proteína muy útil ya que es fácil de detectar y la aplicación de mi laboratorio es para demostrar que ciertas técnicas de espectrometría pueden ser útiles para detectar segregación de proteínas al medio de cultivo. Con GFP es muy fácil de efectuar estos experimentos.