My research entitled as Screening and Analyses of Error Prone PCR Generated Green Fluorescent Protein (GFP) Variants Cloned in pET-BLUE1during this semester doesn’t advance how I expected. We decided to generate new primers. These new primers where expected to amplify the region for GFP inside pGlo beginning exactly in the part that the gene GFP is in. The older primers began in a part before the gene of GFP inside pGlo. This decision was made for amplify precisely the gene of GFP. We did PCR and the primers worked very well. With those primers we did Normal and Mutagenic PCR. Then we purified the products. After that we prepared LB and LB/AMP plaques for the future transformation. This Wednesday we are going to do ligation and transformation. I think that will be the last procedure we are going to do in the lab.  And then we have to establish new goals for the next semester depending in the success of these experiments.