Goals For  This Semester

The goals for this semester are :

1. Screen random mutant GFP molecules to identify those with novel properties.

2. Analyze the sequences of the GFP genes cloned using normal and mutagenic PCR conditions.

3.  Characterize the GFP Flourescence in Escherichia coli.

The work of this semester will be analyzing the results of the last semester. We already generated new variants of GFP in Escherichia coli.

For this semester we are going to work over some techniques:

Transformation
Purification
Ligation
Characterization of the fluorescence of GFP (Not sure of the technique yet)

About the new techniques I’m not sure with precision we are going to do. Today we meet and during this week we are going to be talking with our mentor about it.

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